Catalog No.: PC-72988Not For Human Use, Lab Use Only.
Autophagy activator EN6 (EN6) is a small-molecule in vivo activator of autophagy, covalently targets cysteine 277 in the ATP6V1A subunit of the lysosomal v-ATPase (vacuolar H+-ATPase).
Autophagy activator EN6 (EN6) is a small-molecule in vivo activator of autophagy, covalently targets cysteine 277 in the ATP6V1A subunit of the lysosomal v-ATPase (vacuolar H+-ATPase).
The lysosomal v-ATPase (vacuolar H+-ATPase) activates mTORC1 via the Rag guanosine triphosphatases.
EN6 displaced tetramethylrhodamine-5-iodoacetamide dihydroiodide (IA-rhodamine) labeling from recombinant human ATP6V1A protein with IC50 of 1.7 uM.
EN6-mediated ATP6V1A modification decouples the v-ATPase from the Rags, leading to inhibition of mTORC1 signaling, increased lysosomal acidification and activation of autophagy.
EN6 clears TDP-43 aggregates, a causative agent in frontotemporal dementia, in a lysosome-dependent manner.
