DC-LC3in-D5 is a small molecule covalent modulator of LC3A/B and autophagy inhibitor, binds to LC3B and covalently modifies Lysine 49.
DC-LC3in-D5 selectively reacts with LC3A/B in vitro (IC50=200 nM, FP assays)
DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells, disrupts the ATG7-LC3B interaction and blocks LC3B lipidation.
DC-LC3in-D5 compromises LC3B lipidation in vitro and in HeLa cells, leading to deficiency in the formation of autophagic structures and autophagic substrate degradation.
DC-LC3in-D5 is a powerful tool for autophagy research with little cellular toxicity.
The autophagic ubiquitin-like protein LC3 functions through interactions with LC3-interaction regions (LIRs) of other autophagy proteins, including autophagy receptors, which stands out as a promising protein–protein interaction (PPI) target for the intervention of autophagy.
