FIPI (5-Fluoro-2-indolyl deschlorohalopemide) is a potent, in vivo active phospholipase D (PLD) inhibitor with IC50 of 25 nM for both PLD1 and PLD2.
FIPI inhibits both the hydrolytic and transphosphatidylation activities of PLD1 and PLD2, rapidly blocks in vivo phosphatidic acid (PA) production with subnanomolar potency (IC50=0.5 nM), does inhibit PLD regulation of F-actin cytoskeleton reorganization, cell spreading, and chemotaxis。
FIPI attenuates mercury-induced lipid signaling leading to protection against cytotoxicity in aortic endothelial cells.
