HS38 is a potent and selective DAPK1 and DAPK3 (ZIPK) inhibitor with IC50 of 200 nM for DAPK1 and Kd of 280 nM for ZIPK.
HS38 also inhibits Pim3 (IC50=200 nM), and displays no activity against Src or Abl, little activity against EGFR.
HS38 decreases RLC20 phosphorylation in cells, and decreases contractile force generated in mouse aorta, rabbit ileum, and calyculin A stimulated arterial muscle by decreasing RLC20 and MYPT1 phosphorylation.
HS38 also promotes relaxation in Ca(2+)-sensitized vessels.
