Jun6504 is a specific small molecule inhibitor of Enterovirus D68 (EV-D68) viral 2C inhibitor, shows potent and broad-spectrum antiviral activity with EC50 of 0.25 uM in CPE assay with EV-D68 US/MO/14-18947 in RD cells.
Jun6504 exhibits potent antiviral activities against EV-A71 (EC50 = 0.27 to 0.61 µM) and CVB3 (EC50 = 0.18 to 2.03 µM).
Jun6504 displays broad-spectrum antiviral activity against enteroviruses with EC50 of 0.25 to 0.47 µM against five strains of EV-D68 isolated from the 2014 epidemic.
Jun6504 exhibits dose-dependent inhibition against CVA16, CVA6, poliovirus type 1, and CVB3 in CPE assay with EC50 values from 0.24 to 0.57 µM。
Jun6504 is not cytotoxic in Hela, RD, or Vero cells.
2C-D183V mutation is the predominant contributor to drug resistance, while mutations I112V and D323G confer resistance to a lesser extent.
Jun6504 (50 mg/kg, Vehicle: 90% corn oil + 10% DMSO) is effective at protecting mice from EV-D68 infection-induced paralysis, also reduces viral titers in the spinal cord and the infected quadriceps muscle.