Catalog No.: PC-49024Not For Human Use, Lab Use Only.
PF-4708671 is a potent, specific, cell-permeable inhibitor of S6K1 (p70 ribosomal S6 kinase 1), inhibits the activity of full-length S6K1 in vitro with Ki of 20 nM, and S6K1 isolated from IGF1-stimulated HEK293 cells with IC50 of 160 nM.
PF-4708671 is a potent, specific, cell-permeable inhibitor of S6K1 (p70 ribosomal S6 kinase 1), inhibits the activity of full-length S6K1 in vitro with Ki of 20 nM, and S6K1 isolated from IGF1-stimulated HEK293 cells with IC50 of 160 nM.
PF-4708671 only inhibited very weakly the closely related S6K2 isoform (IC50=56 μM), as well as RSK1 (IC50=4.7 μM) and RSK2 (IC50=9.2 μM) over 20-fold less potently than S6K1.
PF-4708671 prevents the S6K1-mediated phosphorylation of S6 protein in response to IGF-1, while having no effect upon the PMA-induced phosphorylation of substrates of the highly related RSK (p90 ribosomal S6 kinase) and MSK (mitogen- and stress-activated kinase) kinases.
PF-4708671 enhances glucose deprivation-induced cell death via downregulation of anti-apoptotic proteins in MCF-7 breast cancer cells.
PF-4708671 inhibits cell migration in a model of metastasis of triple-negative breast cancer.
