STING agonist BNBC (BNBC) is a human STING specific agonist that activates cGAS-STING pathway in a functional human STING-dependent manner.
BNBC induced ISG54-driven luciferase expression in HepAD38/cGAS-STING/ISG54 cells, did not induce luciferase expression in HepAD38/cGAS-STINGΔC/ISG54Luc cells that express human STING with truncation of carboxyl-terminal domain (CTD).
BNBC also concentration-dependently induced ISG54 driven luciferase expression in HepG2/STING/ISG54 reporter cells lacking cGAS.
BNBC induced a proinflammatory cytokine response and establishment of antiviral state in human foreskin fibroblast cells (THF) in a STING-dependent manner.
Pretreatment of THF cells with BNBC inhibited the replication of Dengue, Yellow fever, and Zika viruses. induced inflammatory cytokine response in human PBMCs and promoted PBMC-derived dendritic cell maturation.
BNBC activated type I and III interferon dominated inflammatory cytokine response in PBMC-derived dendritic cells..
BNBC induced the expression of interferon and other proinflammatory cytokines in HepG2 cells expressing functional human, but not mouse STING.