STO-609 is a selective and cell-permeable inhibitor of CaM-KK, inhibits recombinant CaM-KKα and CaM-KKβ with Ki of 80 and 15 ng/ml.
STO-609 shows no any significant effect on the downstream CaM kinases (CaM-KI and -IV, IC50 >10 ug).
STO-609 suppresses the Ca(2+)-induced activation of CaM-KIV in a dose-dependent manner in transfected HeLa cells.
STO-609 significantly reduces the endogenous activity of CaM-KK in SH-SY5Y neuroblastoma cells (1 ug/ml, 80% inhibition).
