Trichostatin A (TSA) is a potent and specific, irreversible inhibitor of class I/II HDACs with IC50 of 1.8 nM for HDAC. Trichostatin A potently inhibits purified histone deacetylase from wild-type FM3A cells with Ki of 3.4 nM. Trichostatin A markedly prolongs the in vivo half-life of the labile acetyl groups on histones in mouse mammary gland tumor cells, FM3A. Trichostatin A inhibited proliferation of eight breast carcinoma cell lines with IC50 of 26.4-308.1 nM. Trichostatin A exhibits antitumor efficacy and toxicity in vivo in a carcinogen-induced rat mammary cancer model.
