Catalog No.: PC-62201Not For Human Use, Lab Use Only.
20(S)-Hydroxycholesterol is an allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein, binds at a site distinct from the canonical cyclopamine binding site.
20(S)-Hydroxycholesterol (20(S)-OHC) is an allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein, binds at a site distinct from the canonical cyclopamine binding site.
20(S)-OHC activates Hedgehog (Hh) signaling (EC50 =3 uM for induction of Hh reporter gene transcription in in NIH 3T3 cells).
20(S)-OHC induces Smo accumulation in primary cilia.
Physicochemical Properties
M.Wt
402.65
Formula
C27H46O2
Appearance
Solid
CAS No.
Storage
Solide Powder
-20 °C 12 Months; 4°C 6 Months
In Solvent
-80 °C 6 Months; -20°C 6 Months
Shipping
Solubility
10 mM in DMSO
Chemical Name/SMILES
20α-hydroxy Cholesterol
References
1. Nedelcu D, et al. Nat Chem Biol. 2013 Sep;9(9):557-64.
2. Nachtergaele S, et al. Nat Chem Biol. 2012 Jan 8;8(2):211-20.
3. Dwyer JR, et al. J Biol Chem. 2007 Mar 23;282(12):8959-68.
