DI-591 (DI591) is a potent, selective, cell-permeable inhibitor of the DCN1-UBC12 interaction with Ki of 10-12 nM for human DCN1 and DCN2.
DI-591 displays no affinity against DCN3/4/5 (Ki>10 uM).
DI-591 selectively inhibits neddylation of cullin 3 but has no or minimal effect on neddylation of other cullin family members.
DI-591 accumulates NRF2 protein and its transcriptional activation in cells.
