FT385 (FT3967385) is a potent, selective USP30 inhibitor with biochemical IC50 of 1.5 nM.
FT385 (FT3967385) displays high selectivity against a broad panel of USP enzymes, with one exception of plasma membrane-associated USP6.
FT385 engages USP30 in cells at low nanomolar concentrations.
Inhibition of USP30 via FT385 phenocopies USP30 KO in enhancing basal mitophagy and promoting ubiquitylation of TOM20 upon depolarisation, enhances phospho-Ser65 ubiquitin (pUb) levels on mitochondria of SHSY5Y cells.
