Catalog No.: PC-49625Not For Human Use, Lab Use Only.
GSK650394 (GSK 650394) is a potent, competitive inhibitor of serum and glucocorticoid-regulated kinase 1 (SGK1) with IC50 of 62 nM and 103 nM for enzymatic activity of SGK1 and SGK2 repectively, in vitro activity-based scintillation proximity assay (SPA).
GSK650394 (GSK 650394) is a potent, competitive inhibitor of serum and glucocorticoid-regulated kinase 1 (SGK1) with IC50 of 62 nM and 103 nM for enzymatic activity of SGK1 and SGK2 repectively, in vitro activity-based scintillation proximity assay (SPA).
GSK650394 inhibited SGK1-mediated epithelial transport with an IC50 of 0.6 uM in ldosterone-stimulated short circuit current cellular assay (SCC).
GSK650394 displays 60-fold selective for SGK1 over the upstream AGC kinase PDK1 and , >30-fold selectivity over Akt and other related kinases.
GSK650394 also potently inhibits CAMKK2 enzyme with pIC50 of 9.2.
GSK650394 repressed the androgen-mediated enhancement of Nedd4-2 phosphorylation in LNCaP cells, inhibited the androgen-stimulated growth of LNCaP cells with IC50 of 1 uM and completely abrogated androgen-mediated growth at 10 uM.
