GW406108X (GW-406108X, GW108X) is specific inhibitor of Kif15 (Kinesin-12) with IC50 of 0.82 uM in ATPase assays.
GW108X displays a preference for Kif15 over the other mitotic motors, with 30 µM of GW108X inhibiting Kif15 MT gliding by 98.9% ± 0.2% while only inhibiting Eg5 by 19.8% ± 4.3%, HSET by 5.3% ± 2.9% and Kif18A by 4.9%.
GW406108X inhibits the MT-stimulated ATPase and MT gliding activity of Kif15 either by inhibiting ATP binding or by inhibiting MT binding by the motorl.
GW406108X shows a distinct mechanism of action compared with the Kif15 inhibitor, Kif15-IN-1.
