H3B-120 (H3B 120) is a selective allosteric inhibitor of Carbamoyl phosphate synthetase 1 (CPS1) with Ki of 1.4 uM, does not bind to CPS2。
H3B-120 demonstrated complete inhibition of both ATP activity and carbamoyl phosphate synthetase activity of CPS1 with IC50 of 1.5 uM.
H3B-120 blocks pyrimidine labeling in hepatocytes treated with 15N-ammonia, inhibits urea production in a dose-dependent manner, although the cellular potency decreased significantly compared with enzymatic assays.
