L82-G17 is a selective, uncompetitive inhibitor of DNA ligase I, inhibits the third step of the ligation reaction, phosphodiester bond formation.
L82-G17 did not inhibit LigIV.
L82-G17 exhibited increased activity against and selectivity for LigI compared with L82.
Cells lacking nuclear LigIIIα, which can substitute for LigI in DNA replication, were also more sensitive to L82-G17 than isogenic parental cells.
