ML-SI3 (Mucolipin-specific synthetic inhibitor 3) is a synthetic antagonist of transient receptor potential mucolipin 1 (TRPML1), prevents lysosomal calcium efflux and blocks downstream TRPML1-mediated induction of autophagy.
The TRPML1 inhibitor ML-SI3 binds the same cavity as its agonist ML-SA1.
ML-SI3 competes with ML-SA1 to block the channel activity.
ML-SI3 blocks TRPML1 channel activation yet does not inhibit PI(3,5)P2-dependent activation of the channel.
Transient receptor potential mucolipin 1 (TRPML1) regulates lysosomal calcium signaling, lipid trafficking, and autophagy-related processes.
