NGI-1 (ML414) is a potent, cell-permeable oligosaccharyltransferase (OST) inhibitor with IC50 of 1.1 uM.
NGI-1 (ML414) blocks cell-surface localization and signaling of the epidermal growth factor receptor (EGFR) glycoprotein in NSCLC cells.
NGI-1 (ML414) causes cell-cycle arrest accompanied by induction of p21, autofluorescence, and cell morphology changes, all hallmarks of senescence.
NGI-1 (ML414) reduces the production of both OST products (free glycans and N-glycans).
NGI-1 (ML414) is a small molecule chemical probe with N-linked glycosylation inhibitory activity.
