Catalog No.: PC-22648Not For Human Use, Lab Use Only.
Paprotrain is a selective, reversible inhibitor of KIF20A (mitotic kinesin-like protein 2, MKLP-2, MKlp2) with Ki of 3.4 uM, does not inhibit other members of the kinesin superfamily.
Paprotrain is a selective, reversible inhibitor of KIF20A (mitotic kinesin-like protein 2, MKLP-2, MKlp2) with Ki of 3.4 uM, does not inhibit other members of the kinesin superfamily. Paprotrain exhibits IC50 values of 1.35 μM in basal and 0.83 μM in MT-stimulated ATPase assays. Paprotrain is cell permeable and incubation with 10 to 50 μM inhibitor results in binucleated cells, a characteristic phenotype similar to that observed following RNAi-mediated depletion of MKLP-2. Paprotrain induces mitotic spindle defects, perturbs MKLP-2-mediated relocation of the chromosome passenger proteins Aurora B and survivin from the centromeres to the central spindle. Paprotrain does not impair kinesin family member 4 (Kif4)-mediated translocation of protein regulating cytokinesis 1 (PRC1), which emphasizes its specificity for MKLP-2.
