Catalog No.: PC-38235Not For Human Use, Lab Use Only.
SB27012 (SB 27012) is a small molecule PPI inhibitor of the Spike RBD-ACE2 interaction (IC50=7.7 uM in ELISA assays), binds ACE2 (Kd=210 nM) without affecting ACE2 enzymatic activity.
SB27012 (SB 27012) is a small molecule PPI inhibitor of the Spike RBD-ACE2 interaction (IC50=7.7 uM in ELISA assays), binds ACE2 (Kd=210 nM) without affecting ACE2 enzymatic activity.
Using a sandwich ELISA-based screening using the plate-bound RBD (Arg319-Phe541) and extracellular domain of human ACE2 (Gln18-Ser740), SB27012 was identified and affinity determinated.
SB27012 inhibits the ACE2-spike interaction by inducing allosteric changes in ACE2.
SB27012 blocked the SARS-CoV-2 spread with IC50 of 3.3 uM.
SB27012 reduced infection predominantly at early time points, suggesting that SB27012 blocked the entry step of viral infection.
