SPIN1 inhibitor MS31 (MS31) is a potent, selective, and cell-active inhibitor of methyllysine reader protein SPIN1 (spindlin 1) with IC50 of 77 nM.
MS31 potently inhibited binding of trimethyllysine-containing peptides to SPIN1.
MS31 displayed high binding affinity, was highly selective for SPIN1 over other epigenetic readers and writers (G9a/GLP, inactive).
MS31 directly engaged SPIN1 in cells, and was not toxic to nontumorigenic cells.
MS31 selectively binds tudor domain II of SPIN1 (KD=91 nM).
