Spautin-1 is a potent, small molecule inhibitor of autophagy with IC50 of 0.74 uM, inhibits USP10 and USP13 with IC50 of 0.6-0.7 uM, but not USP14.
Spautin-1 promotes cell death under starvation condition and inhibits autophagic cell death, selectively promotes the degradation of Vps34 complexes, reduces the levels of PtdIns3P in cells, but is not a direct inhibitor of class III PI3 kinase activity.
Spautin-1 promotes the degradation of p53 via inhibition of USP10, which can be inhibited in the presence of MG132.
Spautin-1 sensitizes ovarian cancer cells to cisplatin and PARP inhibitor (olaparib).
