Chemical Structure : U4-I05
CAS No.:
956941-31-4
U4-I05
(ZINC000008877313)
Catalog No.: PC-23952Not For Human Use, Lab Use Only.
U4-I05 is a specific small molecule USP4 inhibitor (KD=239 nM) that inhibits USP4 deubiquitinating enzyme activity, inhibits stemness characteristics in CRC cells.
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Purity & Documentation Purity:
>98% (HPLC)
Biological Activity
U4-I05 is a specific small molecule USP4 inhibitor (KD=239 nM) that inhibits USP4 deubiquitinating enzyme activity, inhibits stemness characteristics in CRC cells.
U4-I05 inhibited CD44 protein expression in organoids derived from MC38 cells.
U4-I05 significantly suppressed tumorsphere formation and reduced the proportion of CD44+EpCAM+Lgr5+ cells, with no effects on stemness in cells with USP4 knockdown.
U4-I05 inhibits the protein expression of Twist1 and β-catenin by abolishing the deubiquitinating enzyme activity of USP4, thereby promoting protein degradation.
U4-I05 enhances the sensitivity of tumor cells to oxaliplatin.
U4-I05 exerts a potent anti-tumor effect by downregulating Twist1 and β-catenin expression and enhancing the sensitivity of tumor cells to oxaliplatin in xenograft mice.
Physicochemical Properties
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M.Wt
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498.54
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Formula
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C29H26N2O6
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Appearance
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Solid
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CAS No.
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Storage
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- Solide Powder
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-20°C 12 Months; 4°C 6 Months
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- In Solvent
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-80°C 6 Months; -20°C 6 Months
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Shipping
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Solubility
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10 mM in DMSO
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Chemical Name/SMILES
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(1S,5R)-3-(2-((4-(4-methoxyphenyl)-2-oxo-2H-chromen-7-yl)oxy)acetyl)-1,2,3,4,5,6-hexahydro-8H-1,5-methanopyrido[1,2-a][1,5]diazocin-8-one
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References
1. Li F, et al. J Transl Med. 2025 Jan 24;23(1):114.
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