XL413 (BMS-863233) is a potent, selective, ATP competitive serine/threonine kinase CDC7 inhibitor with IC50 of 3.4 nM. XL413 weakly inhibits CK2, PIM1 with IC50 of 215, 42 nM respectively. XL413 inhibits the cell proliferation (IC50=2685 nM), decreases cell viability (IC50=2142 nM) and elicits the caspase 3/7 activity (EC50=2288 nM) in Colo-205 cells. XL413 also significantly inhibits the anchorage-independent growth of colo-205 in soft agar (IC50=715 nM). XL413 shows cytotoxic effects on tumors, with IC50 of 22.9 µM in HCC1954 cells and 1.1 µM in Colo-205 cells. XL413 demonstrate in vitro CDC7 dependent cell cycle arrest and in vivo tumor growth inhibition in a Colo-205 xenograft model.
