OBI-3424 S-isomer (TH-3424, OBI 3424) is a highly potent DNA alkylating prodrug that is converted by AKR1C3 to a potent DNA-alkylating agent.
OBI-3424 exerted potent cytotoxicity against the H460 lung cancer cell line (IC50 4.0 nM), and potent cytotoxicity, in particular against cell lines derived from T-ALL with high AKR1C3 expression, with IC50 values in the low nM range.
OBI-3424 shows potential anti-leukemic activity against a panel of 19 PDXs representative of B-ALL, T-ALL and ETP-ALL (median IC50=60.3 nM).
OBI-3424 exerted profound in vivo efficacy against T-ALL PDXs, OBI-3424 combined with nelarabine resulted in prolongation of mouse EFS compared with each single agent alone.
1. Richard B. Lock, et al. Abstract LB-B16: The AKR1C3-Activated Prodrug OBI-3424 Exerts Profound In Vivo Efficacy Against Preclinical Models of T-Cell Acute Lymphoblastic Leukemia (T-ALL); a Pediatric Preclinical Testing Consortium Study. AACR 2018.
