SEA0400 (SEA-0400) is a potent and selective Na(+)/Ca(2+) exchanger (NCX) inhibitor, inhibits Na+-dependent Ca2+ uptake in cultured neurons, astrocytes, and microglia with IC50 of 5-33 nM.
SEA0400 is more potent than KB-R7943.
SEA0400 is NCX selective, does not affect the activities of the Na+/H+ exchanger, Na+,K+-ATPase, Ca2+-ATPase.
SEA0400 preferentially inhibits (45)Ca(2+) uptake by NCX1 compared with inhibitions by NCX2, NCX3, and NCKX2.
EA0400 also selectively blocked outward exchange currents from NCX1 transfectants.
SEA0400 protected against hypoxia/reoxygenation-induced cell damage in tubular cells expressing wild-type NCX1.
SEA0400 attenuated dose- dependently paradoxical Ca2+ challenge-induced production of reactive oxygen species, DNA ladder formation, and nuclear condensation in cultured astrocyte.
SEA0400 reduced infarct volumes after a transient middle cerebral artery occlusion in rat cerebral cortex and striatum.