Catalog No.: PC-72305Not For Human Use, Lab Use Only.
ZYF0033 (ZYF 0033) is potent, selective, small-molecule inhibitor of HPK1 (MAP4K1) with IC50 of <10 nM based on the phosphorylation inhibition of MBP protein.
ZYF0033 (ZYF 0033) is potent, selective, small-molecule inhibitor of HPK1 (MAP4K1) with IC50 of <10 nM based on the phosphorylation inhibition of MBP protein.
ZYF0033 successfully decreased the phosphorylation of SLP76 (serine 376), a critical biomarker of T cell and HPK1 activation.
ZYF0033 promoted anticancer immune responses, inhibited tumor growth and caused increased intratumoral infiltration of DCs, NK cells, and CD107a+CD8+ T cells but decreased infiltration of regulatory T cells, PD-1+CD8+ T cells, TIM-3+CD8+ T cells, and LAG-3+CD8+ T cells in the 4T-1 syngeneic mouse model.
ZYF0033 significantly increased the proliferation of CD4+ and CD8+ T cells (Figure S7I) and the secretion of IFN-γ in bone-marrow-derived DCs.
