eIF4A3 inhibitor 53a (eIF4A3-IN-1) is a potent, selective and cell-active eukaryotic initiation factor 4A3 (eIF4A3) inhibitor with IC50 of 0.26 uM, SPR Kd of 43 nM.
eIF4A3 inhibitor 53a displays no activity against eIF4A1, eIF4A2, DHX29 and BRR2 ATPase (IC50>100 uM).
eIF4A3 inhibitor 53a binds to a non-ATP binding site of eIF4A3, exhibits cellular nonsense-mediated RNA decay (NMD) inhibitory activity and represents a novel molecular probe for further study of eIF4A3, the exon junction complex (EJC) and NMD.
