ST-5-002 is a specific small molecule inhibitor of transmembrane 4 L six family member 5 (TM4SF5), inhibits signaling lymphocytic activation molecule family member 7 (SLAMF7) degradation due to TM4SF5 binding and lysosomal translocation.
ST-5-002 reduces colocalization of TM4SF5 and SLAMF7 at lysosomes, leaving more SLAMF7 at the plasma membrane, recovers TM4SF5-dependent decrease in SLAMF7.
TM4SF5 bound SLAMF7 depending on N-glycosylation and caused intracellular trafficking of SLAMF7 from the plasma membrane to lysosomes for degradation.
ST-5-002 inhibits SLAMF7 degradation due to TM4SF5 binding and lysosomal translocation.
ST-5-002 (5 mpk, i.p.) recovered SLAMF7-positive cell populations to block cellular growth.
ST-5-002 abrogates HCC development by increasing the abundance and dispersion of Slamf7-positive cells in liver tissues, recapitulating the phenotype of Tm4sf5-knockout mice and indicating TSI-mediated restoration of NK cell surveillance.