SAG is a small molecule Hedgehog (Hh) pathway agonist with EC50 of 3 nM, specifically binds to the Smoothened (Smo) heptahelical bundle (Kd=59 nM).
SAG effectively activates Shh target gene transcription through the Smoothened (Smo) protein, acts downstream of Ptch1 in the Hh pathway and counteracts cyclopamine inhibition of Smo.
SAG increases Cldn5 expression, decreases endoneurial capillary permeability, and restores thermal algesia to diabetic mice.
SAG inhibits melanin synthesis in melanocytes and pigmentation in a human skin model.
